Hotspot

trnS-GCU-trnG-UCC

LSC · 14732-15740 · 1009 bp

Back to Corydalis

Pi 0.0779

MarkerSeek score 79.70

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 1009
variable_sites: 408
pi: 0.0779
unique_haplotype_count: 43
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	ACGGAAAGAGAGGGATTCGA	ACGAATCACACTTTTACCACT	50.0	38.1	58.2	55.5	550-1151 bp	1.0	62.5
trnS-GCU-trnG-UCC_p2	ACGGAAAGAGAGGGATTCGA	AGAACGAATCACACTTTTACCAC	50.0	39.1	58.2	57.3	553-1154 bp	1.0	61.4
trnS-GCU-trnG-UCC_p3	ACGGAAAGAGAGGGATTCGA	GAACGAATCACACTTTTACCACT	50.0	39.1	58.2	57.3	552-1153 bp	1.0	61.4
trnS-GCU-trnG-UCC_p4	ACGGAAAGAGAGGGATTCGA	AACGAATCACACTTTTACCACT	50.0	36.4	58.2	56.2	551-1152 bp	1.0	60.6
trnS-GCU-trnG-UCC_p5	ACGGAAAGAGAGGGATTCGA	TACCACTAAACTATACCCGC	50.0	45.0	58.2	54.0	536-1137 bp	1.0	58.7