Hotspot

rps15-psaC

IRb · 101425-102391 · 967 bp

Back to Corybas

Pi 0.0395

MarkerSeek score 88.50

Primer available yes

Label rps15-psaC

Feature Metadata

label_name: rps15-psaC
feature_type: igs
region: IRb
length_bp: 967
variable_sites: 90
pi: 0.0395
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 88.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-psaC_p1	TTCTGGGAAAACGTCAACGA	AGCTCGAACACATTGGGTAC	45.0	50.0	57.7	57.9	1001-1219 bp	1.0	87.0
rps15-psaC_p2	TTCTGGGAAAACGTCAACGA	TCGAACACATTGGGTACACC	45.0	50.0	57.7	57.8	998-1216 bp	1.0	86.4
rps15-psaC_p3	TTCTGGGAAAACGTCAACGA	GCTCGAACACATTGGGTACA	45.0	50.0	57.7	58.2	1000-1218 bp	1.0	86.2
rps15-psaC_p4	ACCCGGGAATCAAAAACTCG	AGCTCGAACACATTGGGTAC	50.0	50.0	58.5	57.9	918-1136 bp	1.0	86.2
rps15-psaC_p5	CCCGGGAATCAAAAACTCGT	AGCTCGAACACATTGGGTAC	50.0	50.0	58.5	57.9	917-1135 bp	1.0	86.2