Hotspot

petA-psbJ

LSC · 54280-55010 · 731 bp

Back to Corybas

Pi 0.054

MarkerSeek score 83.00

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 731
variable_sites: 83
pi: 0.054
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 83.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	AGCACGTAAAGAAAAGGGGG	TTTGTCTCTAGCATGACCGC	50.0	50.0	58.1	58.0	1179-1209 bp	1.0	89.1
petA-psbJ_p2	AGCACGTAAAGAAAAGGGGG	TTGGCTGTACCCACTGTTTC	50.0	50.0	58.1	58.0	1452-1482 bp	1.0	87.8
petA-psbJ_p3	TATACCTCCAGGACCGGAAC	TTTGTCTCTAGCATGACCGC	55.0	50.0	57.9	58.0	1107-1137 bp	0.8	76.8
petA-psbJ_p4	TATACCTCCAGGACCGGAAC	TTGGCTGTACCCACTGTTTC	55.0	50.0	57.9	58.0	1380-1410 bp	0.8	75.9
petA-psbJ_p5	TATACCTCCAGGACCGGAAC	GAGAACCGACAAGGGATTCC	55.0	55.0	57.9	58.0	1528-1559 bp	0.8	75.6