Hotspot

ycf4-cemA

LSC · 52037-52393 · 357 bp

Back to Corybas

Pi 0.0503

MarkerSeek score 79.80

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 357
variable_sites: 38
pi: 0.0503
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	GGAAATAAGGGGCCAAGGAG	CAACCATGGGTCGAGTGAAA	55.0	50.0	57.9	58.1	981-1058 bp	1.0	87.7
ycf4-cemA_p2	CCCTGGAATAAATCGTCGCA	CAACCATGGGTCGAGTGAAA	50.0	50.0	58.1	58.1	1086-1135 bp	0.8	77.7
ycf4-cemA_p3	GGATCGCAGGTCTGTTCATT	CAACCATGGGTCGAGTGAAA	50.0	50.0	58.0	58.1	1204-1253 bp	0.8	77.6
ycf4-cemA_p4	GGATCGCAGGTCTGTTCATT	GGGTCGAGTGAAATCCGATC	50.0	55.0	58.0	58.1	1197-1246 bp	0.8	77.3
ycf4-cemA_p5	GGATCGCAGGTCTGTTCATT	GGTCGAGTGAAATCCGATCC	50.0	55.0	58.0	58.1	1196-1245 bp	0.8	77.3