Hotspot

MFP44_pgt017-MFP44_pgt018

LSC · 42662-43355 · 694 bp

Back to Corybas

Pi 0.043
MarkerSeek score 82.70
Primer available yes
Label MFP44_pgt017-MFP44_pgt018

Feature Metadata

label_name
MFP44_pgt017-MFP44_pgt018
feature_type
igs
region
LSC
length_bp
694
variable_sites
67
pi
0.043
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.98
markerseek_score
82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
MFP44_pgt017-MFP44_pgt018_p1 CCTCGTGTCACCAGTTCAAA CCGAGAAAGTCTACGGTTCG 50.0 55.0 58.1 58.1 747-821 bp 1.0 89.6
MFP44_pgt017-MFP44_pgt018_p2 AATCCTCGTGTCACCAGTTC CCGAGAAAGTCTACGGTTCG 50.0 55.0 57.5 58.1 750-824 bp 1.0 86.3
MFP44_pgt017-MFP44_pgt018_p3 TCAGTTGGTAGAGCAGAGGA CCGAGAAAGTCTACGGTTCG 50.0 55.0 57.4 58.1 775-849 bp 1.0 85.0
MFP44_pgt017-MFP44_pgt018_p4 TGGTAGAGCAGAGGACTGAA CCGAGAAAGTCTACGGTTCG 50.0 55.0 57.4 58.1 770-844 bp 1.0 85.0
MFP44_pgt017-MFP44_pgt018_p5 TTGGTAGAGCAGAGGACTGA CCGAGAAAGTCTACGGTTCG 50.0 55.0 57.4 58.1 771-845 bp 1.0 85.0