Hotspot

ycf3-MFP44_pgt014

LSC · 39529-39780 · 252 bp

Back to Corybas

Pi 0.0484
MarkerSeek score 78.90
Primer available yes
Label ycf3-MFP44_pgt014

Feature Metadata

label_name
ycf3-MFP44_pgt014
feature_type
igs
region
LSC
length_bp
252
variable_sites
27
pi
0.0484
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.94
markerseek_score
78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-MFP44_pgt014_p1 TGGCTACAATTGAAGAGGTCT TTCCAATGCTACGCCTTGAA 42.9 45.0 56.6 57.8 318-348 bp 1.0 70.1
ycf3-MFP44_pgt014_p2 TGGCTACAATTGAAGAGGTCTT TTCCAATGCTACGCCTTGAA 40.9 45.0 57.4 57.8 318-348 bp 1.0 67.8
ycf3-MFP44_pgt014_p3 TTGGCTACAATTGAAGAGGTCT TTCCAATGCTACGCCTTGAA 40.9 45.0 57.4 57.8 319-349 bp 1.0 67.8
ycf3-MFP44_pgt014_p4 TGGCTACAATTGAAGAGGTCT AGTTCCAATGCTACGCCTTG 42.9 50.0 56.6 58.5 320-350 bp 1.0 67.2
ycf3-MFP44_pgt014_p5 TGGCTACAATTGAAGAGGTCT CAGTTCCAATGCTACGCCTT 42.9 50.0 56.6 58.5 321-351 bp 1.0 67.2