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MFP44_pgt010-psbD

LSC · 27990-28762 · 773 bp

Back to Corybas

Pi 0.0429

MarkerSeek score 74.30

Primer available yes

Label MFP44_pgt010-psbD

Feature Metadata

label_name: MFP44_pgt010-psbD
feature_type: igs
region: LSC
length_bp: 773
variable_sites: 60
pi: 0.0429
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
MFP44_pgt010-psbD_p1	TGCATGACCAACTCCCTTTC	GCCTAAGACTCCAGCAACTC	50.0	55.0	58.1	58.0	1658-1900 bp	1.0	88.0
MFP44_pgt010-psbD_p2	TGCATGACCAACTCCCTTTC	CACCATCACCGTCTTCGAAT	50.0	50.0	58.1	58.0	1725-1967 bp	0.8	77.1
MFP44_pgt010-psbD_p3	GCATGACCAACTCCCTTTCA	CACCATCACCGTCTTCGAAT	50.0	50.0	58.1	58.0	1966 bp	0.2	47.2
MFP44_pgt010-psbD_p4	GCATGACCAACTCCCTTTCA	GCCTAAGACTCCAGCAACTC	50.0	55.0	58.1	58.0	1899 bp	0.2	47.1
MFP44_pgt010-psbD_p5	GCATGACCAACTCCCTTTCA	CTGCTACACCAAAACTGGGT	50.0	50.0	58.1	58.0	1810 bp	0.2	47.1