Hotspot

rps16-MFP44_pgt002

LSC · 5405-5735 · 331 bp

Back to Corybas

Pi 0.0585
MarkerSeek score 83.50
Primer available yes
Label rps16-MFP44_pgt002

Feature Metadata

label_name
rps16-MFP44_pgt002
feature_type
igs
region
LSC
length_bp
331
variable_sites
43
pi
0.0585
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
83.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-MFP44_pgt002_p1 ATCCACAGCCAATCATGTCC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 427-763 bp 1.0 88.0
rps16-MFP44_pgt002_p2 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 404-740 bp 1.0 87.6
rps16-MFP44_pgt002_p3 CCACAGCCAATCATGTCCTT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 425-761 bp 1.0 87.3
rps16-MFP44_pgt002_p4 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 415-751 bp 1.0 87.0
rps16-MFP44_pgt002_p5 ATCCACAGCCAATCATGTCC CTCGGAGGTTCGAATCCTTC 50.0 55.0 57.9 57.8 431-767 bp 1.0 86.9