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MFP44_pgt001-rps16

LSC · 4030-4456 · 427 bp

Back to Corybas

Pi 0.0511

MarkerSeek score 79.30

Primer available yes

Label MFP44_pgt001-rps16

Feature Metadata

label_name: MFP44_pgt001-rps16
feature_type: igs
region: LSC
length_bp: 427
variable_sites: 47
pi: 0.0511
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
MFP44_pgt001-rps16_p1	CTTTTCCCACATCAGGCACT	GCAAAGACAACGAATTCCGG	50.0	50.0	58.1	58.0	1273-1351 bp	1.0	89.8
MFP44_pgt001-rps16_p2	AAAGCCGAGTACTCTACCGT	GCAAAGACAACGAATTCCGG	50.0	50.0	58.2	58.0	908-987 bp	1.0	89.0
MFP44_pgt001-rps16_p3	AAGCCGAGTACTCTACCGTT	GCAAAGACAACGAATTCCGG	50.0	50.0	58.2	58.0	907-986 bp	1.0	89.0
MFP44_pgt001-rps16_p4	CTTTTCCCACATCAGGCACT	CGCAAAGCAAAGACAACGAA	50.0	45.0	58.1	58.2	1279-1357 bp	1.0	88.6
MFP44_pgt001-rps16_p5	CTTTTCCCACATCAGGCACT	TACGTTTCTAGGGGGCATCT	50.0	50.0	58.1	57.8	1070-1076 bp	0.8	77.0