Hotspot

ycf1

SSC · 120224-125614 · 5391 bp

Back to Coronilla

Pi 0.00816

MarkerSeek score 42.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5391
variable_sites: 44
pi: 0.00816
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTCGTTTAGGAATAGAACTTTTT	CCCCGGAATCGACTTTCATT	26.1	50.0	52.3	58.0	192 bp	1.0	42.5
ycf1_p2	TTTCGTTTAGGAATAGAACTTTT	CCCCGGAATCGACTTTCATT	26.1	50.0	52.3	58.0	193 bp	1.0	42.5
ycf1_p3	TTTTCGTTTAGGAATAGAACTTT	CCCCGGAATCGACTTTCATT	26.1	50.0	52.3	58.0	194 bp	1.0	42.5
ycf1_p4	TTTTTCGTTTAGGAATAGAACTT	CCCCGGAATCGACTTTCATT	26.1	50.0	52.3	58.0	195 bp	1.0	42.5
ycf1_p5	TTCGTTTAGGAATAGAACTTTTT	CCTTTTCTGGTTATCCCCGG	26.1	55.0	52.3	57.9	206 bp	1.0	42.3