Hotspot

ndhD

SSC · 111745-113238 · 1494 bp

Back to Coronilla

Pi 0.00134

MarkerSeek score 48.70

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1494
variable_sites: 2
pi: 0.00134
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TCATTCCATTTTTCCCGAAAACT	TTTGAATGCGGGCTTTTCTG	34.8	45.0	57.2	57.6	1566 bp	0.5	26.1
ndhD_p2	TCATTCCATTTTTCCCGAAAACT	TTCTGGCGCAAGTGTATCTT	34.8	45.0	57.2	57.5	1551 bp	0.5	25.8
ndhD_p3	TCATTCCATTTTTCCCGAAAACT	TTTCTGGCGCAAGTGTATCT	34.8	45.0	57.2	57.5	1552 bp	0.5	25.8
ndhD_p4	TCCATTTTTCCCGAAAACTTTCT	TTTGAATGCGGGCTTTTCTG	34.8	45.0	57.1	57.6	1562 bp	0.5	25.6
ndhD_p5	TCCATTTTTCCCGAAAACTTTCT	TTCTGGCGCAAGTGTATCTT	34.8	45.0	57.1	57.5	1547 bp	0.5	25.3