Hotspot

ndhD-psaC

SSC · 113239-113373 · 135 bp

Back to Coronilla

Pi 0.037

MarkerSeek score 73.80

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 135
variable_sites: 5
pi: 0.037
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	GGAACCGCCTGCTATTGG	GCTTGTCCGACGGATTTCTT	61.1	50.0	58.2	58.6	270 bp	1.0	59.6
ndhD-psaC_p2	GGAACCGCCTGCTATTGG	TGTTGGTTGTAAAAGGTGCG	61.1	45.0	58.2	57.1	295 bp	1.0	57.1
ndhD-psaC_p3	GGAACCGCCTGCTATTGG	GCTTGTCCGACGGATTTCT	61.1	52.6	58.2	57.9	270 bp	1.0	55.0
ndhD-psaC_p4	GGAACCGCCTGCTATTGG	GTTGGTTGTAAAAGGTGCGA	61.1	45.0	58.2	56.9	294 bp	1.0	54.9
ndhD-psaC_p5	GGAACCGCCTGCTATTGG	GTTGTAAAAGGTGCGAATCCG	61.1	47.6	58.2	58.2	290 bp	1.0	54.8