Hotspot

infA-rps8

LSC · 77820-77933 · 114 bp

Back to Coronilla

Pi 0.0175

MarkerSeek score 62.60

Primer available yes

Label infA-rps8

Feature Metadata

label_name: infA-rps8
feature_type: igs
region: LSC
length_bp: 114
variable_sites: 2
pi: 0.0175
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA-rps8_p1	AATGAACCAGAAGCATGCCA	TTGGGAGGTATGGGGATTGT	45.0	50.0	58.1	58.0	292-295 bp	1.0	79.9
infA-rps8_p2	ATGAACCAGAAGCATGCCAA	TTGGGAGGTATGGGGATTGT	45.0	50.0	58.1	58.0	291-294 bp	1.0	79.9
infA-rps8_p3	AGCATGCCAAGTGATTCAGT	TTGGGAGGTATGGGGATTGT	45.0	50.0	57.8	58.0	281-284 bp	1.0	78.8
infA-rps8_p4	CCAGAAGCATGCCAAGTGAT	TTGGGAGGTATGGGGATTGT	50.0	50.0	58.5	58.0	286-289 bp	1.0	76.3
infA-rps8_p5	GAACCAGAAGCATGCCAAGT	TTGGGAGGTATGGGGATTGT	50.0	50.0	58.7	58.0	289-292 bp	1.0	74.5