Hotspot

clpP1-psbB

LSC · 69387-69877 · 491 bp

Back to Coronilla

Pi 0.00424

MarkerSeek score 62.60

Primer available yes

Label clpP1-psbB

Feature Metadata

label_name: clpP1-psbB
feature_type: igs
region: LSC
length_bp: 491
variable_sites: 2
pi: 0.00424
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP1-psbB_p1	CTTTTGGAACACCAATGGGC	AAACGGCCGGGATCATTTAA	50.0	45.0	57.8	57.9	563-569 bp	1.0	80.6
clpP1-psbB_p2	CCAGGACTTCGAAAGGGAAC	AAACGGCCGGGATCATTTAA	55.0	45.0	58.2	57.9	582-588 bp	1.0	80.3
clpP1-psbB_p3	CTTTTGGAACACCAATGGGC	CGATACCAAGGCAAACCCAT	50.0	50.0	57.8	58.2	527-533 bp	1.0	79.9
clpP1-psbB_p4	TTTGGAACACCAATGGGCAT	AAACGGCCGGGATCATTTAA	45.0	45.0	58.3	57.9	561-567 bp	1.0	79.7
clpP1-psbB_p5	TCCAGGACTTCGAAAGGGAA	AAACGGCCGGGATCATTTAA	50.0	45.0	58.3	57.9	583-589 bp	1.0	79.6