Hotspot

trnP-UGG-psaJ

LSC · 63660-64351 · 692 bp

Back to Coronilla

Pi 0.00588

MarkerSeek score 63.80

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 692
variable_sites: 4
pi: 0.00588
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	ACCCGTGACATTTTGTACCC	GTACTTACCACTGGTGCCAC	50.0	55.0	58.1	58.2	786-794 bp	1.0	79.3
trnP-UGG-psaJ_p2	CCCGTGACATTTTGTACCCA	GTACTTACCACTGGTGCCAC	50.0	55.0	58.1	58.2	785-793 bp	1.0	79.3
trnP-UGG-psaJ_p3	ACAGGATTTGAACCCGTGAC	GTACTTACCACTGGTGCCAC	50.0	55.0	58.1	58.2	797-805 bp	1.0	79.2
trnP-UGG-psaJ_p4	CAGGATTTGAACCCGTGACA	GTACTTACCACTGGTGCCAC	50.0	55.0	58.1	58.2	796-804 bp	1.0	79.2
trnP-UGG-psaJ_p5	TGACAGGATTTGAACCCGTG	GTACTTACCACTGGTGCCAC	50.0	55.0	58.1	58.2	799-807 bp	1.0	79.2