Hotspot

petA-psbJ

LSC · 60882-61698 · 817 bp

Back to Coronilla

Pi 0.011

MarkerSeek score 58.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 817
variable_sites: 9
pi: 0.011
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGTGTCCAAGGTCTCTTGTT	ACTGGTATTGCTGTGATCGG	50.0	50.0	57.8	57.7	1001-1003 bp	1.0	76.4
petA-psbJ_p2	CGTGTCCAAGGTCTCTTGTT	CTGGTATTGCTGTGATCGGT	50.0	50.0	57.8	57.7	1000-1002 bp	1.0	76.4
petA-psbJ_p3	CGTGTCCAAGGTCTCTTGTT	TGGTATTGCTGTGATCGGTT	50.0	45.0	57.8	57.2	999-1001 bp	1.0	72.7
petA-psbJ_p4	CGTGTCCAAGGTCTCTTGTT	ATTCCGGATTGGGATCATCC	50.0	50.0	57.8	57.1	952-954 bp	1.0	71.9
petA-psbJ_p5	ACGTGTCCAAGGTCTCTTGT	CTGGTATTGCTGTGATCGGT	50.0	50.0	58.9	57.7	1001-1003 bp	1.0	71.3