Hotspot

accD-psaI

LSC · 57678-57843 · 166 bp

Back to Coronilla

Pi 0.0301

MarkerSeek score 61.60

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 166
variable_sites: 5
pi: 0.0301
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AGCGAGTTATTTCAGCTCCA	TGCCGGAAATACTAGACCCA	45.0	50.0	56.9	58.1	286-290 bp	1.0	71.9
accD-psaI_p2	AGCGAGTTATTTCAGCTCCA	GCCGGAAATACTAGACCCAC	45.0	55.0	56.9	57.8	285-289 bp	1.0	71.0
accD-psaI_p3	AAGCGAGTTATTTCAGCTCCA	TGCCGGAAATACTAGACCCA	42.9	50.0	57.7	58.1	287-291 bp	1.0	69.7
accD-psaI_p4	AAGCGAGTTATTTCAGCTCCA	GCCGGAAATACTAGACCCAC	42.9	55.0	57.7	57.8	286-290 bp	1.0	68.8
accD-psaI_p5	AGCGAGTTATTTCAGCTCCAT	TGCCGGAAATACTAGACCCA	42.9	50.0	57.4	58.1	286-290 bp	1.0	67.9