Hotspot

petN-trnC-GCA

LSC · 32080-32791 · 712 bp

Back to Coronilla

Pi 0.00843

MarkerSeek score 54.60

Primer available yes

Label petN-trnC-GCA

Feature Metadata

label_name: petN-trnC-GCA
feature_type: igs
region: LSC
length_bp: 712
variable_sites: 6
pi: 0.00843
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC-GCA_p1	AGCCCAGGCAAGACTTACTA	TTTCCCCAGTTCAAATCCGG	50.0	50.0	58.0	58.1	768-770 bp	1.0	79.6
petN-trnC-GCA_p2	AGCCCAGGCAAGACTTACTA	TTTATCGTTTTGGCGGCATG	50.0	45.0	58.0	57.7	821-823 bp	1.0	78.0
petN-trnC-GCA_p3	AGCCCAGGCAAGACTTACTA	GGGGGACTGCAAATCCTTTT	50.0	50.0	58.0	58.4	786-788 bp	1.0	77.4
petN-trnC-GCA_p4	TCTTCCCCATACTACGAGGG	TTTCCCCAGTTCAAATCCGG	55.0	50.0	57.6	58.1	819-821 bp	1.0	76.9
petN-trnC-GCA_p5	TACCATTAAAGCAGCCCAGG	TTTCCCCAGTTCAAATCCGG	50.0	50.0	57.6	58.1	780-782 bp	1.0	76.5