Hotspot

trnE-UUC-trnY-GUA

LSC · 30067-30124 · 58 bp

Back to Coronilla

Pi 0.0172

MarkerSeek score 62.40

Primer available yes

Label trnE-UUC-trnY-GUA

Feature Metadata

label_name: trnE-UUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 58
variable_sites: 1
pi: 0.0172
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnY-GUA_p1	AAGTATGCCCCCATCGTCTA	GATCAGCAAATTTGTGGGCC	50.0	50.0	57.9	58.0	237 bp	1.0	79.4
trnE-UUC-trnY-GUA_p2	AAGTATGCCCCCATCGTCTA	GGATCAGCAAATTTGTGGGC	50.0	50.0	57.9	58.0	238 bp	1.0	79.4
trnE-UUC-trnY-GUA_p3	AAGTATGCCCCCATCGTCTA	CATGTCATGGTGGATCAGCA	50.0	50.0	57.9	58.0	249 bp	1.0	79.2
trnE-UUC-trnY-GUA_p4	TCTCTTTCAAGGAGGCAACG	GATCAGCAAATTTGTGGGCC	50.0	50.0	57.8	58.0	202 bp	1.0	78.9
trnE-UUC-trnY-GUA_p5	TCTCTTTCAAGGAGGCAACG	GGATCAGCAAATTTGTGGGC	50.0	50.0	57.8	58.0	203 bp	1.0	78.9