Hotspot

psbD-trnT-GGU

LSC · 28244-29077 · 834 bp

Back to Coronilla

Pi 0.006

MarkerSeek score 62.20

Primer available yes

Label psbD-trnT-GGU

Feature Metadata

label_name: psbD-trnT-GGU
feature_type: igs
region: LSC
length_bp: 834
variable_sites: 5
pi: 0.006
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-trnT-GGU_p1	GAAACGGTCCCTACGTAACC	GGCGTAAGTCATCGGTTCAA	55.0	50.0	58.0	58.3	954-959 bp	1.0	78.1
psbD-trnT-GGU_p2	GAAACGGTCCCTACGTAACC	CCCCTTTAACTCAGCGGTAG	55.0	55.0	58.0	57.7	990-995 bp	1.0	77.9
psbD-trnT-GGU_p3	GAAACGGTCCCTACGTAACC	TGAATCAAATGTGGCCCCTT	55.0	45.0	58.0	57.7	1004-1009 bp	1.0	77.8
psbD-trnT-GGU_p4	GAAACGGTCCCTACGTAACC	TTGAATCAAATGTGGCCCCT	55.0	45.0	58.0	57.7	1005-1010 bp	1.0	77.8
psbD-trnT-GGU_p5	CACAAAAACGAAACGGTCCC	GGCGTAAGTCATCGGTTCAA	50.0	50.0	58.2	58.3	963-968 bp	1.0	76.8