Hotspot

trnK-UUU-rbcL

LSC · 4420-5095 · 676 bp

Back to Coronilla

Pi 0.00904

MarkerSeek score 60.20

Primer available yes

Label trnK-UUU-rbcL

Feature Metadata

label_name: trnK-UUU-rbcL
feature_type: igs
region: LSC
length_bp: 676
variable_sites: 6
pi: 0.00904
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rbcL_p1	AAAGCCGAGTACTCTACCGT	AGTCCTGAATTAGCTGCTGC	50.0	50.0	58.2	58.0	773-785 bp	1.0	80.2
trnK-UUU-rbcL_p2	AAGCCGAGTACTCTACCGTT	AGTCCTGAATTAGCTGCTGC	50.0	50.0	58.2	58.0	772-784 bp	1.0	80.2
trnK-UUU-rbcL_p3	AAGCCGAGTACTCTACCGTT	AATTATCCGTGAAGCGTGCA	50.0	45.0	58.2	58.3	797-809 bp	1.0	78.3
trnK-UUU-rbcL_p4	AAGCCGAGTACTCTACCGTT	ATTATCCGTGAAGCGTGCAA	50.0	45.0	58.2	58.3	796-808 bp	1.0	78.3
trnK-UUU-rbcL_p5	AAGCCGAGTACTCTACCGTT	TATCCGTGAAGCGTGCAAAT	50.0	45.0	58.2	58.3	794-806 bp	1.0	78.3