Hotspot

ycf1

SSC · 127914-133382 · 5469 bp

Back to Cornus

Pi 0.0417
MarkerSeek score 72.40
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5469
variable_sites
874
pi
0.0417
unique_haplotype_count
32
primer_available
yes
alignment_reliability
1.00
markerseek_score
72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 TCCAAAAATGGACCCCAGTC TGAAGCATGGATCGAGATCG 50.0 50.0 57.7 57.6 1161-1176 bp 0.457143 61.0
ycf1_p2 CCAAAAATGGACCCCAGTCT TGAAGCATGGATCGAGATCG 50.0 50.0 57.7 57.6 1160-1175 bp 0.457143 60.9
ycf1_p3 AAACAGACGAACGGGTTCAA TGAAGCATGGATCGAGATCG 45.0 50.0 58.0 57.6 838 bp 0.257143 48.5
ycf1_p4 AACAGACGAACGGGTTCAAT TGAAGCATGGATCGAGATCG 45.0 50.0 57.8 57.6 837 bp 0.257143 46.7
ycf1_p5 AAACAGACGAACGGGTTCAA TCAAGAAAGCAATCCACCCA 45.0 45.0 58.0 57.3 718 bp 0.257143 46.1