Hotspot

ndhC-trnV-UAC

LSC · 53136-54273 · 1138 bp

Back to Cornus

Pi 0.0513
MarkerSeek score 82.50
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1138
variable_sites
230
pi
0.0513
unique_haplotype_count
29
primer_available
yes
alignment_reliability
0.99
markerseek_score
82.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCATATTCGTAAAGCAGAAACA AGAAGGTCTACGGTTCGAGT 31.8 50.0 53.9 57.8 1160-1227 bp 1.0 52.0
ndhC-trnV-UAC_p2 TCATATTCGTAAAGCAGAAACA GAAGGTCTACGGTTCGAGTC 31.8 55.0 53.9 57.5 1159-1226 bp 1.0 52.0
ndhC-trnV-UAC_p3 ATCATATTCGTAAAGCAGAAACA AGAAGGTCTACGGTTCGAGT 30.4 50.0 54.5 57.8 1161-1228 bp 1.0 52.0
ndhC-trnV-UAC_p4 TCATATTCGTAAAGCAGAAACAT AGAAGGTCTACGGTTCGAGT 30.4 50.0 54.5 57.8 1160-1227 bp 1.0 52.0
ndhC-trnV-UAC_p5 TCATATTCGTAAAGCAGAAACA GTTCGAGTCCGTATAGCCCT 31.8 55.0 53.9 58.7 1148-1215 bp 1.0 52.0