Hotspot

trnF-GAA-ndhJ

LSC · 50771-51460 · 690 bp

Back to Cornus

Pi 0.0426
MarkerSeek score 74.50
Primer available yes
Label trnF-GAA-ndhJ

Feature Metadata

label_name
trnF-GAA-ndhJ
feature_type
igs
region
LSC
length_bp
690
variable_sites
121
pi
0.0426
unique_haplotype_count
26
primer_available
yes
alignment_reliability
0.99
markerseek_score
74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-GAA-ndhJ_p1 GGGATAGCTCAGCTGGTAGA TGAAATACAAGATGCTCATTGA 55.0 31.8 58.0 53.8 741-850 bp 1.0 52.0
trnF-GAA-ndhJ_p2 TGATGCATCAGGAATGGTCG TGAAATACAAGATGCTCATTGA 50.0 31.8 58.0 53.8 760-869 bp 1.0 52.0
trnF-GAA-ndhJ_p3 CCTCGTGTCACCAGTTCAAA TGAAATACAAGATGCTCATTGA 50.0 31.8 58.1 53.8 705-814 bp 1.0 52.0
trnF-GAA-ndhJ_p4 CTGGTAGAGCAGAGGACTGA TGAAATACAAGATGCTCATTGA 55.0 31.8 57.9 53.8 729-838 bp 1.0 52.0
trnF-GAA-ndhJ_p5 GGGATAGCTCAGCTGGTAGA ATGAAATACAAGATGCTCATTGA 55.0 30.4 58.0 54.4 742-851 bp 1.0 52.0