Hotspot

ycf3-trnS-GGA

LSC · 46609-47392 · 784 bp

Back to Cornus

Pi 0.0413
MarkerSeek score 75.80
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
784
variable_sites
126
pi
0.0413
unique_haplotype_count
27
primer_available
yes
alignment_reliability
0.98
markerseek_score
75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 CCATTTATTCGCGATCTAGGC ACGGAAAGAGAGGGATTCGA 47.6 50.0 57.0 58.2 863-978 bp 1.0 74.8
ycf3-trnS-GGA_p2 CCATTTATTCGCGATCTAGGC TTCCAATGCTACGCCTTGAA 47.6 45.0 57.0 57.8 813-926 bp 1.0 74.5
ycf3-trnS-GGA_p3 CCATTTATTCGCGATCTAGGC CCCTCGGTAAACAAAAGCCT 47.6 50.0 57.0 58.1 842-950 bp 0.857143 69.5
ycf3-trnS-GGA_p4 CCATTTATTCGCGATCTAGGC ACCCTCGGTAAACAAAAGCC 47.6 50.0 57.0 58.4 843-951 bp 0.857143 67.2
ycf3-trnS-GGA_p5 TCCATTTATTCGCGATCTAGGC CCCTCGGTAAACAAAAGCCT 45.5 50.0 58.4 58.1 843-951 bp 0.857143 66.2