Hotspot

trnT-GGU-psbD

LSC · 33575-34957 · 1383 bp

Back to Cornus

Pi 0.0397
MarkerSeek score 78.20
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1383
variable_sites
226
pi
0.0397
unique_haplotype_count
28
primer_available
yes
alignment_reliability
0.98
markerseek_score
78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG CCGCAACCAGTCATCCATAA 55.0 50.0 58.1 58.0 1296-1548 bp 1.0 91.2
trnT-GGU-psbD_p2 TCAGTGGTAGAGTAACGCCA CCGCAACCAGTCATCCATAA 50.0 50.0 58.1 58.0 1299-1551 bp 1.0 91.0
trnT-GGU-psbD_p3 GGCGTAAGTCATCGGTTCAA CCGCAACCAGTCATCCATAA 50.0 50.0 58.3 58.0 1273-1525 bp 1.0 89.4
trnT-GGU-psbD_p4 GTGGTAGAGTAACGCCATGG GACCGGACCAACCTACAAAA 55.0 50.0 58.1 57.7 1330-1582 bp 1.0 89.4
trnT-GGU-psbD_p5 GTGGTAGAGTAACGCCATGG ACAAAAACGAAACGGTCCCT 55.0 45.0 58.1 58.2 1316-1568 bp 0.971429 88.4