Hotspot

trnE-UUC-trnT-GGU

LSC · 32684-33502 · 819 bp

Back to Cornus

Pi 0.0499
MarkerSeek score 74.10
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
819
variable_sites
147
pi
0.0499
unique_haplotype_count
24
primer_available
yes
alignment_reliability
0.97
markerseek_score
74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 TTTGTGTATGTGCTCCCGAG GAACCGATGACTTACGCCTT 50.0 50.0 57.9 58.0 1454-1606 bp 1.0 91.2
trnE-UUC-trnT-GGU_p2 AGAGAGATGTCCTGAACCCC GAACCGATGACTTACGCCTT 55.0 50.0 58.1 58.0 782-914 bp 0.4 66.8
trnE-UUC-trnT-GGU_p3 GAGAGATGTCCTGAACCCCT GAACCGATGACTTACGCCTT 55.0 50.0 58.1 58.0 781-913 bp 0.4 66.8
trnE-UUC-trnT-GGU_p4 GTCCCCCTTTCACAGAAAGG GAACCGATGACTTACGCCTT 55.0 50.0 58.1 58.0 1289-1323 bp 0.314286 58.1
trnE-UUC-trnT-GGU_p5 GTCCCCCTTTCACAGAAAGG TCTCTGTGGAGGAACGGAAT 55.0 50.0 58.1 58.1 1801-1827 bp 0.314286 57.8