Hotspot

rpoB-trnC-GCA

LSC · 27747-28930 · 1184 bp

Back to Cornus

Pi 0.0382
MarkerSeek score 79.40
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1184
variable_sites
180
pi
0.0382
unique_haplotype_count
29
primer_available
yes
alignment_reliability
1.00
markerseek_score
79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 CCCTCATTTCCATCCCGAAG CCGGATTTGAACTGGGGAAA 55.0 50.0 58.0 58.1 1120-1300 bp 1.0 91.1
rpoB-trnC-GCA_p2 CTCATTTCCATCCCGAAGCA CCGGATTTGAACTGGGGAAA 50.0 50.0 58.0 58.1 1118-1298 bp 1.0 91.0
rpoB-trnC-GCA_p3 CCCTCATTTCCATCCCGAAG AAAAGGATTTGCAGTCCCCC 55.0 50.0 58.0 58.4 1102-1282 bp 1.0 88.9
rpoB-trnC-GCA_p4 CTCATTTCCATCCCGAAGCA AAAAGGATTTGCAGTCCCCC 50.0 50.0 58.0 58.4 1100-1280 bp 1.0 88.8
rpoB-trnC-GCA_p5 TCCCTCATTTCCATCCCGAA CCGGATTTGAACTGGGGAAA 50.0 50.0 58.4 58.1 1121-1301 bp 1.0 88.1