Hotspot

atpH-atpI

LSC · 14502-15470 · 969 bp

Back to Cornus

Pi 0.04
MarkerSeek score 76.50
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
969
variable_sites
148
pi
0.04
unique_haplotype_count
24
primer_available
yes
alignment_reliability
0.98
markerseek_score
76.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 TACCTTGACCAACTCCAGGT TTTTTGCAACTTTAGCCGCG 50.0 45.0 57.9 58.5 1069-1313 bp 0.942857 84.7
atpH-atpI_p2 ATAACGGAAGCGGCAGAAAT TTTTTGCAACTTTAGCCGCG 45.0 45.0 58.0 58.5 1013-1257 bp 0.914286 84.1
atpH-atpI_p3 ATAACGGAAGCGGCAGAAAT TAGGTGAATCCATGGAGGGT 45.0 50.0 58.0 57.4 986-1230 bp 0.914286 83.6
atpH-atpI_p4 TACCTTGACCAACTCCAGGT TAGGTGAATCCATGGAGGGT 50.0 50.0 57.9 57.4 1042-1286 bp 0.914286 83.0
atpH-atpI_p5 AATAACGGAAGCGGCAGAAA TTTTTGCAACTTTAGCCGCG 45.0 45.0 58.2 58.5 1014-1258 bp 0.914286 83.0