Hotspot

ycf1

IRb · 114055-117966 · 3912 bp

Back to Cornukaempferia

Pi 0.0123

MarkerSeek score 51.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRb
length_bp: 3912
variable_sites: 48
pi: 0.0123
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCAAAATTGTACGATTCACAA	ATGGGAGGTGGACGAATTTC	28.6	50.0	52.0	57.6	245-270 bp	1.0	48.0
ycf1_p2	TCAAAATTGTACGATTCACAA	AATCTATGGGAGGTGGACGA	28.6	50.0	52.0	57.5	250-275 bp	1.0	48.0
ycf1_p3	TCAAAATTGTACGATTCACAA	ATCTATGGGAGGTGGACGAA	28.6	50.0	52.0	57.5	249-274 bp	1.0	48.0
ycf1_p4	TCAAAATTGTACGATTCACAA	TCTATGGGAGGTGGACGAAT	28.6	50.0	52.0	57.5	248-273 bp	1.0	48.0
ycf1_p5	TCAAAATTGTACGATTCACAA	GGGAGGTGGACGAATTTCTT	28.6	50.0	52.0	57.5	243-268 bp	1.0	48.0