Hotspot

ndhF-rpl32

SSC · 120152-120831 · 680 bp

Back to Cornukaempferia

Pi 0.0126
MarkerSeek score 70.30
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
680
variable_sites
8
pi
0.0126
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GTCGGAATAAGTAGAAGCCCA AGAAAAAGCCTTTACTGCAGC 47.6 42.9 57.2 57.3 871-904 bp 1.0 57.0
ndhF-rpl32_p2 GTCGGAATAAGTAGAAGCCCA GAAAAAGCCTTTACTGCAGCT 47.6 42.9 57.2 57.3 870-903 bp 1.0 57.0
ndhF-rpl32_p3 TGTCGGAATAAGTAGAAGCCC AGAAAAAGCCTTTACTGCAGC 47.6 42.9 57.2 57.3 872-905 bp 1.0 57.0
ndhF-rpl32_p4 TGTCGGAATAAGTAGAAGCCC GAAAAAGCCTTTACTGCAGCT 47.6 42.9 57.2 57.3 871-904 bp 1.0 57.0
ndhF-rpl32_p5 GTCGGAATAAGTAGAAGCCCA AAAAAGCCTTTACTGCAGCT 47.6 40.0 57.2 56.1 869-902 bp 1.0 55.2