Hotspot

rps18-rpl20

LSC · 72183-72385 · 203 bp

Back to Cornukaempferia

Pi 0.0246

MarkerSeek score 66.70

Primer available yes

Label rps18-rpl20

Feature Metadata

label_name: rps18-rpl20
feature_type: igs
region: LSC
length_bp: 203
variable_sites: 5
pi: 0.0246
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18-rpl20_p1	TCCCCCGAACTCGAATTACT	AAACAGAATTCCCCGGAGTG	50.0	50.0	58.1	57.8	265 bp	1.0	78.8
rps18-rpl20_p2	CCCCCGAACTCGAATTACTC	AAACAGAATTCCCCGGAGTG	55.0	50.0	57.8	57.8	264 bp	1.0	78.1
rps18-rpl20_p3	TCCCCCGAACTCGAATTACT	AACGAAACAGAATTCCCCGG	50.0	50.0	58.1	58.5	269 bp	1.0	76.6
rps18-rpl20_p4	TCCCCCGAACTCGAATTACT	GAAACAGAATTCCCCGGAGT	50.0	50.0	58.1	57.5	266 bp	1.0	76.4
rps18-rpl20_p5	CCCCCGAACTCGAATTACTC	AACGAAACAGAATTCCCCGG	55.0	50.0	57.8	58.5	268 bp	1.0	76.0