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psbE-petL

LSC · 68339-69568 · 1230 bp

Back to Cornukaempferia

Pi 0.00746

MarkerSeek score 64.80

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1230
variable_sites: 9
pi: 0.00746
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AAGCAGCCAGTAGAAAACCG	50.0	50.0	57.9	58.5	1377-1550 bp	1.0	77.6
psbE-petL_p2	CAACCCGCAATGAATAGGGA	GCAGCCAGTAGAAAACCGAA	50.0	50.0	57.9	58.5	1375-1548 bp	1.0	77.6
psbE-petL_p3	ACTGACGAATAACCAACCCG	AAGCAGCCAGTAGAAAACCG	50.0	50.0	57.9	58.5	1390-1563 bp	1.0	77.3
psbE-petL_p4	ACTGACGAATAACCAACCCG	GCAGCCAGTAGAAAACCGAA	50.0	50.0	57.9	58.5	1388-1561 bp	1.0	77.2
psbE-petL_p5	ACGAATAACCAACCCGCAAT	AAGCAGCCAGTAGAAAACCG	45.0	50.0	58.2	58.5	1386-1559 bp	1.0	76.6