Hotspot

trnS-GGA-rps4

LSC · 47775-48048 · 274 bp

Back to Cornukaempferia

Pi 0.0471

MarkerSeek score 62.10

Primer available yes

Label trnS-GGA-rps4

Feature Metadata

label_name: trnS-GGA-rps4
feature_type: igs
region: LSC
length_bp: 274
variable_sites: 9
pi: 0.0471
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.70
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GGA-rps4_p1	ATGTAGGAGAGATGGCCGAG	ATTATTCCCGTCAGACTTGA	55.0	40.0	58.4	53.8	303-386 bp	1.0	49.2
trnS-GGA-rps4_p2	TTCGAATCCCTCTCTTTCCG	ATTATTCCCGTCAGACTTGA	50.0	40.0	57.0	53.8	231-314 bp	1.0	46.6
trnS-GGA-rps4_p3	GGGTTCGAATCCCTCTCTTT	ATTATTCCCGTCAGACTTGA	50.0	40.0	56.9	53.8	234-317 bp	1.0	46.6
trnS-GGA-rps4_p4	GGTTCGAATCCCTCTCTTTCC	ATTATTCCCGTCAGACTTGA	52.4	40.0	58.1	53.8	233-316 bp	1.0	46.6
trnS-GGA-rps4_p5	TATGTAGGAGAGATGGCCGA	ATTATTCCCGTCAGACTTGA	50.0	40.0	57.0	53.8	304-387 bp	1.0	46.1