Hotspot

trnT-GGU-psbD

LSC · 34241-35525 · 1285 bp

Back to Cornukaempferia

Pi 0.025

MarkerSeek score 61.90

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1285
variable_sites: 31
pi: 0.025
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	ATTGACCCGCTTCACCTTTT	AGCACCATGAAGAGCAACAA	45.0	45.0	58.0	58.0	1852-1905 bp	1.0	83.8
trnT-GGU-psbD_p2	AAAATTGACCCGCTTCACCT	CACCGTCACCATCTTCGAAT	45.0	50.0	58.0	58.0	2183-2236 bp	1.0	83.3
trnT-GGU-psbD_p3	AAATTGACCCGCTTCACCTT	CACCGTCACCATCTTCGAAT	45.0	50.0	58.0	58.0	2182-2235 bp	1.0	83.3
trnT-GGU-psbD_p4	AATTGACCCGCTTCACCTTT	CACCGTCACCATCTTCGAAT	45.0	50.0	58.0	58.0	2181-2234 bp	1.0	83.3
trnT-GGU-psbD_p5	ATTGACCCGCTTCACCTTTT	CACCGTCACCATCTTCGAAT	45.0	50.0	58.0	58.0	2180-2233 bp	1.0	83.3