Hotspot

trnE-UUC-trnT-GGU

LSC · 33296-34168 · 873 bp

Back to Cornukaempferia

Pi 0.0213

MarkerSeek score 66.60

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 873
variable_sites: 18
pi: 0.0213
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	TCCTTTCGTAGTACCCCCAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.1	58.0	985-1012 bp	1.0	82.5
trnE-UUC-trnT-GGU_p2	TCCTTTCGTAGTACCCCCAG	CCATGGCGTTACTCTACCAC	55.0	55.0	58.1	58.1	964-991 bp	1.0	82.1
trnE-UUC-trnT-GGU_p3	TCCTTTCGTAGTACCCCCAG	TGGCGTTACTCTACCACTGA	55.0	50.0	58.1	58.1	961-988 bp	1.0	81.9
trnE-UUC-trnT-GGU_p4	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	949-976 bp	1.0	81.7
trnE-UUC-trnT-GGU_p5	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	928-955 bp	1.0	81.6