Hotspot

petN-psbM

LSC · 30149-31434 · 1286 bp

Back to Cornukaempferia

Pi 0.00864
MarkerSeek score 64.70
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
1286
variable_sites
11
pi
0.00864
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 AGTATGGGGAAGGAGTGGAC TGCTACTGCACTGTTCATTCT 55.0 42.9 58.1 57.6 1390-1530 bp 1.0 69.1
petN-psbM_p2 GTATGGGGAAGGAGTGGACT TGCTACTGCACTGTTCATTCT 55.0 42.9 58.1 57.6 1389-1529 bp 1.0 69.1
petN-psbM_p3 ATGGGGAAGGAGTGGACTTT TGCTACTGCACTGTTCATTCT 50.0 42.9 58.2 57.6 1387-1527 bp 1.0 68.1
petN-psbM_p4 ATTGTAAGTCTCGCTTGGGC TGCTACTGCACTGTTCATTCT 50.0 42.9 58.3 57.6 1449-1589 bp 1.0 67.9
petN-psbM_p5 TGGGGAAGGAGTGGACTTTA TGCTACTGCACTGTTCATTCT 50.0 42.9 57.6 57.6 1386-1526 bp 1.0 66.6