Hotspot

rpoC1

LSC · 20302-23127 · 2826 bp

Back to Corispermum

Pi 0.000531

MarkerSeek score 56.40

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: LSC
length_bp: 2826
variable_sites: 3
pi: 0.000531
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	ACTCGAATTCCACAAGTCCG	ACGATCTTTGGCTCTGGAAC	50.0	50.0	57.9	57.9	2969-2972 bp	1.0	78.7
rpoC1_p2	CTCGAATTCCACAAGTCCGA	ACGATCTTTGGCTCTGGAAC	50.0	50.0	57.6	57.9	2968-2971 bp	1.0	76.4
rpoC1_p3	ACTCGAATTCCACAAGTCCG	CGATCTTTGGCTCTGGAACT	50.0	50.0	57.9	57.6	2968-2971 bp	1.0	76.3
rpoC1_p4	CTCGAATTCCACAAGTCCGA	CGATCTTTGGCTCTGGAACT	50.0	50.0	57.6	57.6	2967-2970 bp	1.0	74.1
rpoC1_p5	ACTCGAATTCCACAAGTCCG	ACTACGATCTTTGGCTCTGG	50.0	50.0	57.9	57.0	2972-2975 bp	1.0	71.7