Hotspot

rpl22

LSC · 83378-84049 · 672 bp

Back to Corispermum

Pi 0.00223

MarkerSeek score 32.10

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 672
variable_sites: 3
pi: 0.00223
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 32.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	CCCGACTCTTTAGTTGTCCG	TGGGTAGAGCCGGATCTAAG	55.0	55.0	58.0	58.0	1663 bp	1.0	80.3
rpl22_p2	CCGACTCTTTAGTTGTCCGG	TGGGTAGAGCCGGATCTAAG	55.0	55.0	58.0	58.0	1662 bp	1.0	80.3
rpl22_p3	CCCGACTCTTTAGTTGTCCG	AGCTTAACACAAAGGCGGAA	55.0	45.0	58.0	58.0	1316 bp	1.0	80.2
rpl22_p4	CCGACTCTTTAGTTGTCCGG	AAGCTTAACACAAAGGCGGA	55.0	45.0	58.0	58.0	1316 bp	1.0	80.2
rpl22_p5	CCGACTCTTTAGTTGTCCGG	AGCTTAACACAAAGGCGGAA	55.0	45.0	58.0	58.0	1315 bp	1.0	80.2