Hotspot

ndhA

SSC · 117637-119818 · 2182 bp

Back to Corispermum

Pi 0.0029

MarkerSeek score 44.70

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2182
variable_sites: 12
pi: 0.0029
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	GGTAAACGACCCAGAGCAAT	AAGAGTTGAAGCCCCAAAGG	50.0	50.0	57.9	58.0	2921-2925 bp	1.0	79.6
ndhA_p2	ACGAAAACGCTCTGATGTGA	AAGAGTTGAAGCCCCAAAGG	45.0	50.0	57.9	58.0	2646-2650 bp	1.0	79.3
ndhA_p3	ACGAAAACGCTCTGATGTGA	GGGGCTTATCAGGTCCAATG	45.0	55.0	57.9	58.0	2980-2984 bp	1.0	79.2
ndhA_p4	CACGAAAACGCTCTGATGTG	AAGAGTTGAAGCCCCAAAGG	50.0	50.0	57.8	58.0	2647-2651 bp	1.0	78.8
ndhA_p5	CACGAAAACGCTCTGATGTG	GGGGCTTATCAGGTCCAATG	50.0	55.0	57.8	58.0	2981-2985 bp	1.0	78.6