Hotspot

ycf2-trnL-CAA

IRb · 92282-92933 · 652 bp

Back to Corispermum

Pi 0.00613

MarkerSeek score 39.80

Primer available yes

Label ycf2-trnL-CAA

Feature Metadata

label_name: ycf2-trnL-CAA
feature_type: igs
region: IRb
length_bp: 652
variable_sites: 8
pi: 0.00613
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 39.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-trnL-CAA_p1	CCGGAAAGATATGTGGCCAT	AAATGGTAGACACGCGAGAC	50.0	50.0	57.7	58.0	743 bp	1.0	78.6
ycf2-trnL-CAA_p2	CCGGAAAGATATGTGGCCAT	TGAAATGGTAGACACGCGAG	50.0	50.0	57.7	58.0	745 bp	1.0	78.6
ycf2-trnL-CAA_p3	CCCATTCCTTAGCCGGAAAG	AAATGGTAGACACGCGAGAC	55.0	50.0	58.3	58.0	755 bp	1.0	78.2
ycf2-trnL-CAA_p4	CCCATTCCTTAGCCGGAAAG	TGAAATGGTAGACACGCGAG	55.0	50.0	58.3	58.0	757 bp	1.0	78.2
ycf2-trnL-CAA_p5	CCGGAAAGATATGTGGCCAT	GCCTTGGTGGTGAAATGGTA	50.0	50.0	57.7	58.1	755 bp	1.0	78.0