Hotspot

rbcL-accD

LSC · 56752-57369 · 618 bp

Back to Corispermum

Pi 0.00351

MarkerSeek score 61.10

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 618
variable_sites: 4
pi: 0.00351
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GAGGCTAGCAAATGGAGTCC	TCGAATTGAACCACCGTTGT	55.0	45.0	58.0	58.0	826 bp	1.0	79.7
rbcL-accD_p2	GCTGCTTGTGAGGTATGGAA	TCGAATTGAACCACCGTTGT	50.0	45.0	57.9	58.0	796 bp	1.0	79.1
rbcL-accD_p3	GAGGCTAGCAAATGGAGTCC	TAGAGCTTTCTTGCCCCCTA	55.0	50.0	58.0	57.7	802 bp	1.0	78.0
rbcL-accD_p4	TTCGTGAGGCTAGCAAATGG	TCGAATTGAACCACCGTTGT	50.0	45.0	58.3	58.0	831 bp	1.0	77.9
rbcL-accD_p5	ATTCCCAGCAATGGATACCG	TCGAATTGAACCACCGTTGT	50.0	45.0	57.7	58.0	761 bp	1.0	77.9