Hotspot

trnF-GAA-ndhJ

LSC · 48350-49035 · 686 bp

Back to Corispermum

Pi 0.00219

MarkerSeek score 58.00

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 686
variable_sites: 3
pi: 0.00219
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.0	58.1	827-828 bp	1.0	79.7
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	791-792 bp	1.0	79.3
trnF-GAA-ndhJ_p3	AAGGTGAATGAATGGTCGGG	AGTTGGATAGGATGGCCCTT	50.0	50.0	57.9	58.1	844-845 bp	1.0	78.7
trnF-GAA-ndhJ_p4	CTGGTAGAGCAGAGGACTGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	57.9	58.1	815-816 bp	1.0	78.6
trnF-GAA-ndhJ_p5	TGAATGGTCGGGATAGCTCA	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.2	58.1	836-837 bp	1.0	78.0