Hotspot

trnD-GUC-trnY-GUA

LSC · 29875-30291 · 417 bp

Back to Corispermum

Pi 0.0052
MarkerSeek score 40.40
Primer available yes
Label trnD-GUC-trnY-GUA

Feature Metadata

label_name
trnD-GUC-trnY-GUA
feature_type
igs
region
LSC
length_bp
417
variable_sites
4
pi
0.0052
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-trnY-GUA_p1 GCGGTGCTCTAACCAATTGA TGGCAATATGTCTACGCTGG 50.0 50.0 58.6 57.8 488 bp 1.0 74.3
trnD-GUC-trnY-GUA_p2 GCGGTGCTCTAACCAATTGA GGCAATATGTCTACGCTGGT 50.0 50.0 58.6 57.8 487 bp 1.0 74.3
trnD-GUC-trnY-GUA_p3 GCGGTGCTCTAACCAATTGA GGGGACGGACTGTAAATTCG 50.0 55.0 58.6 58.4 509 bp 1.0 73.4
trnD-GUC-trnY-GUA_p4 GCGGTGCTCTAACCAATTGA GGGACGGACTGTAAATTCGT 50.0 50.0 58.6 57.6 508 bp 1.0 73.2
trnD-GUC-trnY-GUA_p5 GCGGTGCTCTAACCAATTGA CGGACTGTAAATTCGTTGGC 50.0 50.0 58.6 57.5 504 bp 1.0 72.1