Hotspot

rps16-trnQ-UUG

LSC · 5977-6586 · 610 bp

Back to Corispermum

Pi 0.00246

MarkerSeek score 53.90

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 610
variable_sites: 3
pi: 0.00246
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	CCACAACGGATCATGTCCTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.9	58.1	700-705 bp	1.0	79.4
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	679-684 bp	1.0	79.2
rps16-trnQ-UUG_p3	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	690-695 bp	1.0	78.7
rps16-trnQ-UUG_p4	CCACAACGGATCATGTCCTT	TAAGGCATCGGGTTTTGGTC	50.0	50.0	57.9	58.2	730-735 bp	1.0	78.6
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	TAAGGCATCGGGTTTTGGTC	45.0	50.0	58.1	58.2	709-714 bp	1.0	78.4