Hotspot

rps2

Genome · 57893-58603 · 711 bp

Back to Corethrodendron

Pi 0

MarkerSeek score 36.50

Primer available yes

Label rps2

Feature Metadata

label_name: rps2
feature_type: gene
region: Genome
length_bp: 711
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 36.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2_p1	TTGCACAATTTTGTAATGGT	AAAGAGAAGGTTCCGTTGGA	30.0	45.0	52.1	56.7	906-907 bp	1.0	40.1
rps2_p2	TTGCACAATTTTGTAATGGT	AAGAGAAGGTTCCGTTGGAA	30.0	45.0	52.1	56.7	905-906 bp	1.0	40.1
rps2_p3	TTTGCACAATTTTGTAATGGT	AAAGAGAAGGTTCCGTTGGA	28.6	45.0	53.0	56.7	907-908 bp	1.0	40.1
rps2_p4	TTTGCACAATTTTGTAATGGT	AAGAGAAGGTTCCGTTGGAA	28.6	45.0	53.0	56.7	906-907 bp	1.0	40.1
rps2_p5	TCTATGAATTTCCCACCAAAA	AAGAGAAGGTTCCGTTGGAA	33.3	45.0	53.0	56.7	865-866 bp	1.0	40.1