Hotspot

rpl20

Genome · 41859-42221 · 363 bp

Back to Corethrodendron

Pi 0.00275

MarkerSeek score 55.40

Primer available yes

Label rpl20

Feature Metadata

label_name: rpl20
feature_type: gene
region: Genome
length_bp: 363
variable_sites: 1
pi: 0.00275
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20_p1	CTACCACCTTCCCGGAGTTA	AGGAAGGATAAAAGAATAACTGA	55.0	30.4	58.1	52.8	465 bp	1.0	40.2
rpl20_p2	CTACCACCTTCCCGGAGTTA	AGGAAGGATAAAAGAATAACTGAAAGA	55.0	29.6	58.1	56.7	465 bp	1.0	40.2
rpl20_p3	CTACCACCTTCCCGGAGTTA	ACTAGGAAGGATAAAAGAATAACTGA	55.0	30.8	58.1	55.7	468 bp	1.0	40.2
rpl20_p4	CTACCACCTTCCCGGAGTTA	GACTAGGAAGGATAAAAGAATAACTGA	55.0	33.3	58.1	56.7	469 bp	1.0	40.2
rpl20_p5	CTACCACCTTCCCGGAGTTA	AGACTAGGAAGGATAAAAGAATAACTG	55.0	33.3	58.1	56.7	470 bp	1.0	40.2