Hotspot

psbE

Genome · 37663-37914 · 252 bp

Back to Corethrodendron

Pi 0

MarkerSeek score 22.90

Primer available yes

Label psbE

Feature Metadata

label_name: psbE
feature_type: gene
region: Genome
length_bp: 252
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 22.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE_p1	GAACAGCCAACCATCGTACT	GGAATCGATCCCCCTTTGAC	50.0	55.0	57.9	58.0	358 bp	1.0	79.0
psbE_p2	AACAGCCAACCATCGTACTG	GGAATCGATCCCCCTTTGAC	50.0	55.0	58.2	58.0	357 bp	1.0	78.2
psbE_p3	GTGAACAGCCAACCATCGTA	GGAATCGATCCCCCTTTGAC	50.0	55.0	58.2	58.0	360 bp	1.0	78.2
psbE_p4	TGAACAGCCAACCATCGTAC	GGAATCGATCCCCCTTTGAC	50.0	55.0	58.2	58.0	359 bp	1.0	78.2
psbE_p5	GAACAGCCAACCATCGTACT	TCGATCCCCCTTTGACTGTA	50.0	50.0	57.9	57.8	354 bp	1.0	77.3