Hotspot

rps2-rpoC2

Genome · 58604-58882 · 279 bp

Back to Corethrodendron

Pi 0.0036

MarkerSeek score 54.90

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: Genome
length_bp: 279
variable_sites: 1
pi: 0.0036
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	GTGCCATTCGAGGATTCCAT	CCGGTCAAGGCAACATAACA	50.0	50.0	58.0	58.5	493-494 bp	1.0	76.3
rps2-rpoC2_p2	AAGGTGCCATTCGAGGATTC	CCGGTCAAGGCAACATAACA	50.0	50.0	58.0	58.5	496-497 bp	1.0	76.2
rps2-rpoC2_p3	TGCCATTCGAGGATTCCATT	CCGGTCAAGGCAACATAACA	45.0	50.0	57.3	58.5	492-493 bp	1.0	70.7
rps2-rpoC2_p4	GGCCAAAATGAACTCCTGCT	CCGGTCAAGGCAACATAACA	50.0	50.0	58.7	58.5	460-461 bp	1.0	70.7
rps2-rpoC2_p5	GTGCCATTCGAGGATTCCAT	CCCGGTCAAGGCAACATAAC	50.0	55.0	58.0	59.2	494-495 bp	1.0	70.6