Hotspot

psbE-petL

Genome · 37915-38836 · 922 bp

Back to Corethrodendron

Pi 0.00108

MarkerSeek score 59.40

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: Genome
length_bp: 922
variable_sites: 1
pi: 0.00108
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TGACAAATAACCAACCCGCA	ACTGATAAAAAGACCTGCGGT	45.0	42.9	58.0	57.6	1109 bp	1.0	68.6
psbE-petL_p2	CAACCCGCAATGAATAGGGA	ACTGATAAAAAGACCTGCGGT	50.0	42.9	57.9	57.6	1098 bp	1.0	68.3
psbE-petL_p3	AGAACGTTCTCCTGTGCTTC	ACTGATAAAAAGACCTGCGGT	50.0	42.9	57.8	57.6	1021 bp	1.0	67.5
psbE-petL_p4	GTTCTCCTGTGCTTCCAGAC	ACTGATAAAAAGACCTGCGGT	55.0	42.9	58.2	57.6	1016 bp	1.0	67.1
psbE-petL_p5	ACCAACCCGCAATGAATAGG	ACTGATAAAAAGACCTGCGGT	50.0	42.9	58.2	57.6	1100 bp	1.0	66.8